Human Cystatin A Antibody

Detection of Human Cystatin A by Western Blot.

Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1407) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Cystatin A by Flow Cytometry

Expression of CD163 and CD169 in PRRSV-infected cells. Freshly isolated cells were infected with the PRRSV isolate RFLP-144 at an MOI of 2. At 24 hpi, cells were stained with antibodies against CD163, CD169, and PRRSV-N protein and analyzed by flow cytometry. Cells were first gated for PRRSV+ populations and subsequently analyzed for CD163 and CD169 expression within the PRRSV+ population. (A) Representative gating strategy. (B) Percentage of different cell populations within the PRRSV+ cells. (C) MFI of PRRSV-N protein expression in different cell populations. Experiments were performed using cells from three different pigs. ** p ≤ 0.01. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fmicb.2024.1505900/full), licensed under a CC-BY license. Not internally tested by R&D Systems.