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Human Cystatin A Antibody

Catalog #: AF1407
1 Citation Datasheet / COA / SDS
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AF1407
AF1407-SP

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Detection of Human Cystatin A by Western Blot.
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Human Cystatin A Antibody Summary

Species Reactivity
Human
Specificity
Detects Cystatin A in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Cystatin A, recombinant human (rh) Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin F, rhCystatin S, rhCystatin SA and rhCystatin SN is observed.  
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cystatin A
Ile2-Phe98
Accession # P01040
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
THP-1 human acute monocytic leukemia cell line
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Cystatin A (Catalog # 1407-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Cystatin A by Western Blot. Western Blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Goat Anti-Human Cystatin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1407) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin A at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin A

Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).

References
  1. Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
  2. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  3. Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
  4. Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
  5. Sinha, A.A. et al. (2002) Cancer 94:3141.
Entrez Gene IDs
1475 (Human)
Alternate Names
AREI; CSTA; cystatin A (stefin A); Cystatin A; cystatin AS; cystatin-A; Stefin A; STF1; STF1Cystatin-AS; STFA; STFAStefin-A

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Citation for Human Cystatin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Single Amino Acid Substitution in Porcine Reproductive and Respiratory Syndrome Virus Glycoprotein 2 Significantly Impairs Its Infectivity in Macrophages
    Authors: J Chaudhari, RA Leme, K Durazo-Mar, S Sillman, AM Workman, HLX Vu
    Viruses, 2022-12-18;14(12):.

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