Human Cytohesin-1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF4807V Datasheet / COA / SDS

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AF4807V-100UG

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All Cytohesin-1 Antibodies

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Human Cytohesin-1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Cytohesin-1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cytohesin-1
Met1-Tyr102
Accession # Q15438

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cytohesin-1

Cytohesin-1, previously called B2-1 or PSCD1 (pleckstrin homology, Sec7 and coiled-coil domain-containing protein 1), is a 398 amino acid (aa), 47 kDa member of the cytohesin family of guanine nucleotide exchange factors. The Sec7 domain (aa 62‑244) binds the integrin beta 2 subunit cytoplasmic domain, activating integrins via inside-out signaling, thus implicated in chemotaxis, adhesion, and cell spreading. The region of human Cytohesin-1 used as an immunogen shares 97% and 96% aa identity with mouse and rat Cytohesin-1, and 67% and 69% aa identity with human cytohesins 2 and 3, respectively. Although ubiquitously expressed, Cytohesin-1 is primarily found in immune system cells, and was cloned from a natural killer (NK) cell minus T helper cell line Jurkat library.

Entrez Gene IDs

9267 (Human); 19157 (Mouse); 116691 (Rat)

Alternate Names

B2-1; CYTH1; cytoadhesin 1; cytohesin 1; Cytohesin1; Cytohesin-1; D17S811ECYTOHESIN-1; FLJ34050; FLJ41900; homolog of secretory protein SEC7; PH, SEC7 and coiled-coil domain-containing protein 1; pleckstrin homology, Sec7 and coiled-coil domains 1; PSCD1; SEC7 homolog B2-1; SEC7