Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

R&D Systems | Catalog # AF5545

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cytosolic Sulfotransferase 1E1/SULT1E1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888

Specificity

Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhSulfotransferase 2A1 and rhSulfotransferase 4A1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Cytosolic Sulfotransferase 1E1/SULT1E1 (Catalog # 5545-ST), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line

Reviewed Applications

Read 2 reviews rated 4 using AF5545 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).

References

  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
 
       

      Alternate Names

      EST-1, STE, SULT1E1

      Entrez Gene IDs

      6783 (Human); 20860 (Mouse); 25355 (Rat)

      Gene Symbol

      SULT1E1

      UniProt

      P49888

      Additional Cytosolic Sulfotransferase 1E1/SULT1E1 Products

      Product Documents for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

      Certificate of Analysis

      To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

      Note: Certificate of Analysis not available for kit components.

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      Product Specific Notices for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

      For research use only

      Citations for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

      Customer Reviews for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (2)

      4 out of 5
      2 Customer Ratings
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      Showing  1 - 2 的 2 reviews Showing All
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      • Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
        Name: Hua Jiang
        Application: Western Blot
        Sample Tested: Liver
        Species: Mouse
        Verified Customer | Posted 10/15/2021
        WB for SULT1E1 (Goat)
        Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody AF5545
        Bio-Techne Response
        This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
      • Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
        Name: Hua Jiang
        Application: Western Blot
        Sample Tested: Liver tissue and Kidney tissue
        Species: Human and Mouse
        Verified Customer | Posted 06/01/2016
        Primary ab dilution 1:2000 (Maybe working with 1:4000) 40 ug protein per lane
        Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody AF5545

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