Human DEC2 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF5226S Datasheet / COA / SDS

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AF5226S-100UG

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All DEC2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DEC2 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEC2 direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DEC1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DEC2
Ala101-Gly287
Accession # Q9C0J9

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DEC2

DEC-2 (Differentially expressed in chondrocytes protein 2; also bHLHB3, Sharp-1 and bHLHe41) is a 54-56 kDa Class B member of the bHLH superfamily of proteins. It utilizes both DNA binding and protein-protein interaction to mediate E-box transcriptional repression. Physiologically, DEC-2 is proposed to be a regulator of the sleep cycle, particularly during periods of sleep deprivation. It also interacts with HIF-1 alpha neutralizing its activation of the VEGF gene during hypoxia. Human DEC-2 is 482 amino acids (aa) in length. It contains a DNA binding motif (aa 45-57), an HLH region (aa 58-100), an Orange domain that may impart specificity to protein-protein interactions (aa 131-166) and a Gly/Ala-rich region (aa 297-431). DEC-2 forms homodimers, and heterodimers with MyoD and E-proteins. There is one potential alternate start site at Met280. Over aa 101‑287, there is less than 60% aa identity between human and mouse DEC-2.

Long Name

Differentially expressed in chondrocytes protein 2

Entrez Gene IDs

79365 (Human); 79362 (Mouse); 117095 (Rat)

Alternate Names

basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix family, member e41; BHLHB3; BHLHB3class E basic helix-loop-helix protein 41; BHLHE41; Class B basic helix-loop-helix protein 3; DEC2; DEC2Enhancer-of-split and hairy-related protein 1; Differentially expressed in chondrocytes protein 2; SHARP-1; SHARP1hDEC2