Human DIAPH-2 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB6806V Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6806V-100UG

Save 15% on Select RUO Reagents. See Details.

All DIAPH-2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DIAPH-2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DIAPH-2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 699142

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DIAPH-2
Val314-Gln540
Accession # O60879

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DIAPH-2

DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.

Long Name

Diaphanous Homolog 2

Entrez Gene IDs

1730 (Human); 54004 (Mouse); 317189 (Rat)

Alternate Names

DIA2; DIAdiaphanous (Drosophila, homolog) 2; DIAPH2; DIAPH-2; diaphanous homolog 2 (Drosophila); Diaphanous-related formin-2; diaphorase-2; DRF2; POF; POF2; POF2FLJ11167; protein diaphanous homolog 2