Human DNA-PKcs Antibody

R&D Systems | Catalog # AF3415

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DNA-PKCS
Thr3844-Trp4127
Accession # P78527

Specificity

Detects human DNA-PKCS in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DNA-PKcs Antibody

Detection of Human DNA-PKcs antibody by Western Blot.

Detection of Human DNA-PKcs by Western Blot.

Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human DNA-PKcs Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3415) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DNA-PKcs at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DNA-PKcs Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNA-PKcs

DNA-dependent protein kinase catalytic subunit (DNA-PKCS) is a member of the PI3-kinase related kinase (PIKK) superfamily. DNA-PKCS is a serine/threonine kinase that, along with the DNA-binding proteins Ku70/Ku80, binds to regions of DNA double-strand breaks and is involved in double-strand break repair and VDJ recombination.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Entrez Gene IDs

5591 (Human); 19090 (Mouse); 360748 (Rat)

Gene Symbol

PRKDC

UniProt

P78527

Additional DNA-PKcs Products

Product Documents for Human DNA-PKcs Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DNA-PKcs Antibody

For research use only

Related Research Areas

DNA Repair

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