Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Bat, Camelid, Ferret, Primate

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Blocking

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DPPIV/CD26 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
Asp34-Pro766
Accession # Q53TN1

Specificity

Detects human DPPIV/CD26 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DPPIV/CD26 Antibody

Detection of Human DPPIV/CD26 antibody by Western Blot.

Detection of Human DPPIV/CD26 by Western Blot.

Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human DPPIV/CD26 antibody by Western Blot.

Detection of Human DPPIV/CD26 by Western Blot.

Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DPPIV/CD26 antibody in Human PBMCs by Immunocytochemistry (ICC).

DPPIV/CD26 in Human PBMCs.

DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

DPPIV/CD26 antibody in Human Psoriatic Skin by Immunohistochemistry (IHC-P).

DPPIV/CD26 in Human Psoriatic Skin.

DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human DPPIV/CD26 antibody by Simple WesternTM.

Detection of Human DPPIV/CD26 by Simple WesternTM.

Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human DPPIV/CD26 by Western Blot

Detection of Human DPPIV/CD26 by Western Blot

Expression of different markers in the eight human colon cancer cell lines analyzed. (A) Western blot analysis of EpCAM, LGR5, CD26, E-cadherin and vimentin expression in total cell extracts from the eight cell lines (20 μg of protein in each line). Data shown are representative of three experiments. (B) E-cadherin and EpCAM expression analysis by immunofluorescence in HT-29 and Caco-2 cells. (C) CD44 and CD26 expression analysis by immunofluorescence in HT-29 and Caco-2 cells. (D) LGR5 expression analysis by immunofluorescence in DLD-1 and Caco-2 cells. Nuclei were stained with DAPI. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31285270), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human DPPIV/CD26 by Immunocytochemistry/ Immunofluorescence

Detection of Human DPPIV/CD26 by Immunocytochemistry/ Immunofluorescence

Comparison of histone modifier sets between two hepatic cell cultures.(A) Differentiation scheme of hESC towards Hepatocyte-like cells (Hep-like). (B) Hep-like cells were generated from hESC and stained with antibodies specific for DPP4 and albumin. Nuclei were stained with the DNA dye H-33342 (blue). Scale bars: 100 µm. (C) RT-qPCR data from three independent experiments for hepatic (ALB, CYP3A, CYP7A1, DPP4, HNF4, MET) and neuronal (TH, DCX, TUBB3) markers. Relative gene expression was calculated using hESC as a calibrator and a set of three reference genes (HPRT, RPL13A, GAPDH). (D) Transcript levels of epigenetic modifiers were measured by RT-qPCR in human liver (huHep), Hep-like islets (Hep-like) and embryonic stem cells (hESC). Data for huHep and Hep-like are indicated as relative change compared to hESC (as reference cell). For comparative display, a scatter plot was constructed so that differentially expressed genes that show pos. association (between Hep-like and huHep) are found in red fields, and those that differed in the sense of regulation fall into blue fields. Values of >10 were set to 10. For quadrant count ratio analysis (QCR) only expression values >2 or <−2 were included. (E) The data measured in D were plotted as heat map, sorted according to rel. huHep expression levels. Transcripts that were >2-fold higher expressed in tissue than in hESC are marked in red, >2-fold lower expression is marked in blue. The color scale ranges from a fold regulation of −20 (dark blue) to +20 (dark red). Measures of variance and p-values are indicated in the supplemental material, genes not regulated significantly (vs. hESC) are displayed as “n.s.”. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0102035), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human DPPIV/CD26 by Immunocytochemistry/ Immunofluorescence

Detection of Human DPPIV/CD26 by Immunocytochemistry/ Immunofluorescence

Comparison of histone modifier sets between two hepatic cell cultures.(A) Differentiation scheme of hESC towards Hepatocyte-like cells (Hep-like). (B) Hep-like cells were generated from hESC and stained with antibodies specific for DPP4 and albumin. Nuclei were stained with the DNA dye H-33342 (blue). Scale bars: 100 µm. (C) RT-qPCR data from three independent experiments for hepatic (ALB, CYP3A, CYP7A1, DPP4, HNF4, MET) and neuronal (TH, DCX, TUBB3) markers. Relative gene expression was calculated using hESC as a calibrator and a set of three reference genes (HPRT, RPL13A, GAPDH). (D) Transcript levels of epigenetic modifiers were measured by RT-qPCR in human liver (huHep), Hep-like islets (Hep-like) and embryonic stem cells (hESC). Data for huHep and Hep-like are indicated as relative change compared to hESC (as reference cell). For comparative display, a scatter plot was constructed so that differentially expressed genes that show pos. association (between Hep-like and huHep) are found in red fields, and those that differed in the sense of regulation fall into blue fields. Values of >10 were set to 10. For quadrant count ratio analysis (QCR) only expression values >2 or <−2 were included. (E) The data measured in D were plotted as heat map, sorted according to rel. huHep expression levels. Transcripts that were >2-fold higher expressed in tissue than in hESC are marked in red, >2-fold lower expression is marked in blue. The color scale ranges from a fold regulation of −20 (dark blue) to +20 (dark red). Measures of variance and p-values are indicated in the supplemental material, genes not regulated significantly (vs. hESC) are displayed as “n.s.”. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0102035), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of DPPIV/CD26 by Western Blot

Detection of DPPIV/CD26 by Western Blot

Nuclear Translocation of Antitumor CD26 mAbs in Cancer Cells. (D) Jurkat/mock or Jurkat/CD26 cells incubated with biotin-labeled control IgG1 or 1F7 for 1 hour. Nuclear (Nuc)&membrane (Mem) extracts of these cells pulled-down with Neutravidin,&then subjected to immunoblot analysis using streptavidin or antibodies to CD26 or Na+/K+ ATPase (membrane marker). HC, heavy chain; LC, light chain. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23638030), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human DPPIV/CD26 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate and psoriatic skin

Simple Western

2 µg/mL
Sample: LoVo human colorectal adenocarcinoma cell line

Western Blot

0.2 µg/mL
Sample: Human peripheral blood mononuclear cells (PBMC) and LoVo human colorectal adenocarcinoma cell line

Reviewed Applications

Read 6 reviews rated 4.7 using AF1180 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Long Name

Dipeptidyl-peptidase IV

Alternate Names

CD26, DPP4

Entrez Gene IDs

1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)

Gene Symbol

DPP4

UniProt

Q53TN1

Additional DPPIV/CD26 Products

Product Documents for Human DPPIV/CD26 Antibody

Certificate of Analysis

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Product Specific Notices for Human DPPIV/CD26 Antibody

For research use only

Citations for Human DPPIV/CD26 Antibody

Customer Reviews for Human DPPIV/CD26 Antibody (6)

4.7 out of 5
6 Customer Ratings
5 Stars
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4 Stars
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Customer Images

Showing  1 - 5 的 6 reviews Showing All
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  • Human DPPIV/CD26 Antibody
    Name: Ali Baity
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 01/30/2023
    Human DPPIV/CD26 Antibody AF1180
  • Human DPPIV/CD26 Antibody
    Name: Techung Lee
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 10/09/2020
    Serum-starved HepG2 cells were treated with 100 ng/ml phosphoglucose isomerase (PGI) in minimal essential medium for 2 days, following which cells were harvested and analyzed by Western blotting using the CD26 antibody (1000 fold dilution). The study shows that PGI induces the expression of CD26.
    Human DPPIV/CD26 Antibody AF1180
  • Human DPPIV/CD26 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Small intestine
    Species: Human
    Verified Customer | Posted 01/21/2020
    IHC-paraffin working dilution: 1ug/mL with Bond Refine polymer Detection rabbit anti-goat IgG as a Linker antibody epitope retrieval: basic (BOND ER2)
    Human DPPIV/CD26 Antibody AF1180
  • Human DPPIV/CD26 Antibody
    Name: Sam Connell
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 02/22/2019
    Human DPPIV/CD26 Antibody AF1180
  • Human DPPIV/CD26 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Heparin Plasma
    Species: Mouse
    Verified Customer | Posted 08/11/2016
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23638030
    Species: Human
    Verified Customer | Posted 01/05/2015

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