Human E6AP/UBE3A Antibody (1077332)

R&D Systems | Catalog # MAB11498

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # 1077332
View all E6AP/UBE3A Primary Antibodies »

Product Specifications

Immunogen

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A 
Met1-Leu875
Accession # Q05086

Specificity

Detects human E6AP/UBE3A in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E6AP/UBE3A Antibody (1077332)

Detection of Human E6AP/UBE3A by Western Blot.

Western Blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E6AP/UBE3A at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human E6AP/UBE3A by Simple WesternTM.

Simple Western shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for E6AP/UBE3A at approximately 106 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11498). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human E6AP/UBE3A Antibody (1077332)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: K562 human chronix myelogenous leukemia cell line

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

References

  1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
  2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
  3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
  4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
  5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
  6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Entrez Gene IDs

7337 (Human); 22215 (Mouse); 361585 (Rat)

Gene Symbol

UBE3A

UniProt

Q05086 (Human)

Additional E6AP/UBE3A Products

Product Documents for Human E6AP/UBE3A Antibody (1077332)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human E6AP/UBE3A Antibody (1077332)

For research use only

Related Research Areas

E3 Ubiquitin Ligases

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail