Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ENPP-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2
Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product.
Accession # Q13822 (ENPP-2) P22413 (ENPP-1)

Specificity

Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ENPP‑1 Antibody

Detection of Human ENPP-1 antibody by Western Blot.

Detection of Human ENPP‑1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, human kidney tissue, and human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ENPP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6136) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP-1 at approximately 130-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human ENPP‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample:  HepG2 human hepatocellular carcinoma cell line, human liver tissue, human kidney tissue, and human thyroid tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-1

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1).

References

  1. Goldfine, I.D. et al. (2010) Endocrine Reviews. 29:62.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. 99:9445.
 

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Alternate Names

ENPP1, Ly-41 Antigen, M6S1, NPP1, NPPS, PCA1, PDNP1

Entrez Gene IDs

5167 (Human); 18605 (Mouse)

Gene Symbol

ENPP1

UniProt

Q13822 (ENPP-2) P22413 (ENPP-1)

Additional ENPP-1 Products

Product Documents for Human ENPP‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ENPP‑1 Antibody

For research use only

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