Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ERK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ERK3
Met344-Asn721
Accession # Q16659

Specificity

Detects endogenous human ERK3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ERK3 Antibody

Detection of Human ERK3 antibody by Western Blot.

Detection of Human ERK3 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human ERK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK3 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human ERK3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK3

Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination.

Long Name

Extracellular Signal-regulated Kinase 3

Alternate Names

MAPK6, p97MAPK

Entrez Gene IDs

5597 (Human); 50772 (Mouse); 58840 (Rat)

Gene Symbol

MAPK6

UniProt

Q16659

Additional ERK3 Products

Product Documents for Human ERK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human ERK3 Antibody

For research use only

Related Research Areas

GDNF Family
Intracellular Kinases

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