Human EVI-1 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB7506N Datasheet / COA / SDS

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FAB7506N-100UG

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All EVI-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EVI-1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human EVI-1 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 702319

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: EVI-1

Ectopic virus integration site 1 (EVI1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI1 shares 94% aa sequence identity with mouse and rat EVI1.

Long Name

Ecotropic Viral Integration Site 1

Entrez Gene IDs

2122 (Human); 14013 (Mouse); 294924 (Rat)

Alternate Names

AML1-EVI-1 fusion protein; AML1-EVI-1; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI1; EVI-1; EVI1MDS1-EVI1; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1 and EVI1 complex locus; MDS1; MECOM; MGC163392; MGC97004; Myelodysplasia syndrome 1 protein; myelodysplasia syndrome 1; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; PRDM3; zinc finger protein Evi1