Human FAM3A Antibody

Detection of FAM3A by Immunocytochemistry/ Immunofluorescence

Fam3a rescues impaired commitment of Stat3 knockout (KO) muscle stem cells (MuSCs) in vitro and in vivo. a Immunofluorescence analysis and quantification of myogenin expression in MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 3 independent experiments). Scale bar 25 μm. b Immunofluorescence analysis and quantification of myogenin expression in control and Stat3 KO MuSCs cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). Scale bar 25 μm. c–e Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of Stat3 KO MuSCs cultured in growth conditions for 3 days in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). The Cell Mito Stress Test was performed using the Seahorse XFp Analyzer. f Scheme of the experimental design for the intramuscular Fam3a treatment in vivo. g, h Immunofluorescence analysis and quantification of Pax7- and myogenin-positive cells in transversal sections of the tibialis anterior muscles of Pax7-CreERWT;Stat3f/f and Pax7-CreER;Stat3f/f male mice 6 days after barium chloride injury. Vehicle or 1 μg of Fam3a recombinant protein was injected intramuscularly 1 day after inducing injury (n = 3–4 animals). Scale bar 25 μm. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, **p < 0.01, ****p < 0.0001) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30996264), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FAM3A by Immunohistochemistry

Fam3a rescues impaired commitment of Stat3 knockout (KO) muscle stem cells (MuSCs) in vitro and in vivo. a Immunofluorescence analysis and quantification of myogenin expression in MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 3 independent experiments). Scale bar 25 μm. b Immunofluorescence analysis and quantification of myogenin expression in control and Stat3 KO MuSCs cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). Scale bar 25 μm. c–e Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of Stat3 KO MuSCs cultured in growth conditions for 3 days in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). The Cell Mito Stress Test was performed using the Seahorse XFp Analyzer. f Scheme of the experimental design for the intramuscular Fam3a treatment in vivo. g, h Immunofluorescence analysis and quantification of Pax7- and myogenin-positive cells in transversal sections of the tibialis anterior muscles of Pax7-CreERWT;Stat3f/f and Pax7-CreER;Stat3f/f male mice 6 days after barium chloride injury. Vehicle or 1 μg of Fam3a recombinant protein was injected intramuscularly 1 day after inducing injury (n = 3–4 animals). Scale bar 25 μm. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, **p < 0.01, ****p < 0.0001) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30996264), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FAM3A by Immunohistochemistry

Fam3a rescues impaired commitment of Stat3 knockout (KO) muscle stem cells (MuSCs) in vitro and in vivo. a Immunofluorescence analysis and quantification of myogenin expression in MuSCs infected with shCt or shFam3a coding lentiviruses and cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 3 independent experiments). Scale bar 25 μm. b Immunofluorescence analysis and quantification of myogenin expression in control and Stat3 KO MuSCs cultured in growth conditions for 72 h in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). Scale bar 25 μm. c–e Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of Stat3 KO MuSCs cultured in growth conditions for 3 days in the presence or absence of recombinant Fam3a (1000 ng/ml) (n = 5 independent experiments). The Cell Mito Stress Test was performed using the Seahorse XFp Analyzer. f Scheme of the experimental design for the intramuscular Fam3a treatment in vivo. g, h Immunofluorescence analysis and quantification of Pax7- and myogenin-positive cells in transversal sections of the tibialis anterior muscles of Pax7-CreERWT;Stat3f/f and Pax7-CreER;Stat3f/f male mice 6 days after barium chloride injury. Vehicle or 1 μg of Fam3a recombinant protein was injected intramuscularly 1 day after inducing injury (n = 3–4 animals). Scale bar 25 μm. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, **p < 0.01, ****p < 0.0001) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30996264), licensed under a CC-BY license. Not internally tested by R&D Systems.