Human FHL2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF4758N Datasheet / COA / SDS

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AF4758N-100UG

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Human FHL2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FHL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human FHL1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FHL2
Lys167-Ile279
Accession # Q14192

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FHL2

FHL2 (Four-and-a-half LIM domains protein 2; also SLIM3 and DRAL) is a 30‑32 kDa member of the four-and-a half class of the LIM domain-only protein family. It shows a tissue-specific expression pattern (myocardium; skeletal muscle; prostatic epithelium), and is noted for its strong transactivation of the androgen receptor (AR). Human FHL2 is 279 amino acids (aa) in length. It contains four distinct LIM domains (aa 40‑92; 101‑153; 162‑212; 221‑275) that all bind to the AR. There are two potential alternate start sites (Met115 and Met110) and one splice variant that shows a 99 aa substitution for aa 53‑279. Over aa 167‑279, human FHL2 is 95% and 96% aa identical to canine and mouse FHL2, respectively.

Long Name

Four and a Half LIM Domains Protein 2

Entrez Gene IDs

2274 (Human); 14200 (Mouse); 63839 (Rat)

Alternate Names

AAG11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; DRALAAG11; FHL2; FHL-2; four and a half LIM domains 2; four and a half LIM domains protein 2; four and a half LIM-domain protein 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM3; SLIM-3; SLIM3aging-associated gene 11