Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FLI1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FLI1
Ser13-Pro112
Accession # Q01543

Specificity

Detects human FLI1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FLI1 Antibody

Detection of Human FLI1 antibody by Western Blot.

Detection of Human FLI1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 0.5 µg/mL of Human FLI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FLI1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FLI1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FLI1

FLI1 (Friend leukemia virus integration site 1; also ERGB) is a member of the ETS family of transcription factors. Although its predicted MW is 51 kDa. FLI1 is expressed by megakaryocytes, macrophages, B cells and embryonic endothelial cells. It is a transcriptional activator that regulates the genes for Tie-2, GpIIb, MPL and TGF-beta RII. Human FLI1 is 452 amino acids (aa) in length. It contains one PNT domain that is involved in heterotypic interactions (aa 112-198) and an ETS DNA‑binding domain (aa 281-361). There are two potential splice variants. One shows an alternative start site at Met34, while another contains a 10 aa substitution for aa 1‑76. There are a series of naturally occurring 62-68 kDa chimeric proteins (EWS/FLI1) in tumors that result from a chromosomal translocation involving the EWS and FLI1 genes. One creates a 499 aa chimera that includes aa 1-265 of EWS and 220-452 of FLI1. Over aa 13-112, human FLI1 shares 98% aa identity with mouse FLI1.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Entrez Gene IDs

2313 (Human); 14247 (Mouse); 315532 (Rat)

Gene Symbol

FLI1

UniProt

Q01543 (Human)

Additional FLI1 Products

Product Documents for Human FLI1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FLI1 Antibody

For research use only

Related Research Areas

ETS Family Transcription Factors

Citations for Human FLI1 Antibody

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