Human FoxP4 Antibody

R&D Systems | Catalog # AF6854

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FoxP4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FoxP4
Pro549-Ser680
Accession # Q8IVH2

Specificity

Detects human FoxP4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP2, rhFoxP3, and rhCTBP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human FoxP4 Antibody

Detection of Human FoxP4 antibody by Western Blot.

Detection of Human FoxP4 by Western Blot.

Western blot shows lysates of COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line either mock transfected, transfected with human FoxP1, transfected with human FoxP2, transfected with human FoxP3, or transfected with human FoxP4. PVDF membrane was probed with 1:1000 dilution of Sheep Anti-Human FoxP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6854) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. For additional reference, the membrane was co-probed with an Anti-Actin Antibody. A specific band was detected for FoxP4 at approximately 75 kDa and Actin at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions.Image courtesy of Dr. Alison Banham, Ms. Linden Lyne, and Dr. Paola Bignone, The University of Oxford, Oxford, UK.

Applications for Human FoxP4 Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: COS‑7 African green monkey SV40 transformed kidney fibroblast-like cell line transfected with human FoxP4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FoxP4

FoxP4 (Forkhead box protein P4; also FKHLA) is a 70-80 kDa member of the winged helix transcription factor gene family, FoxP subfamily of proteins. It is a transcriptional repressor that is strongly expressed in embryonic gut and lung epithelium, and in neurons both pre-and postnatally. Postnatally, it is found in cerebellar Purkinje cells. During development, it is found in hippocampal hilus, proliferating cells of the ventricular zone, and neurons of the basolateral nucleus of the amygdala. Human FoxP4 is 680 amino acids (aa) in length. It contains a large poly-Gln region (aa 65-219), a C2H2 Zn-finger domain (aa 307-332), a Leu-zipper dimerization motift (aa 349-370) and a forkhead DNA binding domain (aa 467-559). Phosphorylation occurs at Ser360. FoxP4 forms homodimers, and heterodimers with FoxP1 and FoxP2; it does not bind CTBP1. One potential splice form shows a deletion of aa 384-395. Over aa 549-680, human and mouse FoxP4 share 89% aa identity.

Long Name

Forkhead Box P4

Alternate Names

FKHLA

Entrez Gene IDs

116113 (Human); 74123 (Mouse); 363185 (Rat)

Gene Symbol

FOXP4

UniProt

Q8IVH2 (Human)

Additional FoxP4 Products

Product Documents for Human FoxP4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FoxP4 Antibody

For research use only

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