Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 961416
View all FSH beta Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human FSH beta
Asn19-Glu129
Accession # P01225

Specificity

Detects human FSH beta in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human FSH  beta Antibody

Detection of Human FSH beta antibody by Western Blot.

Detection of Human FSH beta by Western Blot.

Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FSH beta Monoclonal Antibody (Catalog # MAB43101) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for FSH beta at approximately 18-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FSH  beta Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human pituitary gland tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FSH beta

Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland.  Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.

References

  1. Van de Wiel, D.F. et al. (1998) J. Mol. Endocrinol. 20:83.
  2. Rathnam, P. and B.B. Saxena (1975) J. Biol. Chem. 250:6735.

Long Name

Follicle Stimulating Hormone, beta

Alternate Names

Follitropin, FSHB

Entrez Gene IDs

2488 (Human); 14308 (Mouse); 25447 (Rat)

Gene Symbol

FSHB

UniProt

P01225

Additional FSH beta Products

Product Documents for Human FSH  beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FSH  beta Antibody

For research use only

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