Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 425037
View all Fucosyltransferase 3/5 (FUT3/5) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fucosyltransferase 3
Arg35-Thr361
Accession # P21217

Specificity

Detects human Fucosyltransferase 3/5 (FUT3/5) in Western blots. The amino acid sequences of recombinant human FUT3 and recombinant human FUT5 are 91% identical.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human Fucosyltransferase 3/5 (FUT3/5) Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)

Reviewed Applications

Read 2 reviews rated 3.5 using MAB4068 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fucosyltransferase 3/5 (FUT3/5)

Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha -4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa 1 to 15), a transmembrane region (aa 16 to 34) and an ectodomain (aa 35 to 361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken.

Alternate Names

CD174, FT3B, FucT-III, LE, Les

Entrez Gene IDs

2525 (Human)

Gene Symbol

FUT3

UniProt

P21217

Additional Fucosyltransferase 3/5 (FUT3/5) Products

Product Documents for Human Fucosyltransferase 3/5 (FUT3/5) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Fucosyltransferase 3/5 (FUT3/5) Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Customer Reviews for Human Fucosyltransferase 3/5 (FUT3/5) Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human Fucosyltransferase 3/5 (FUT3/5) Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human Fucosyltransferase 3/5 (FUT3/5) Antibody MAB4068
  • Human Fucosyltransferase 3/5 (FUT3/5) Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019

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