Gab2 (GRB2-associated binding protein 2) is a 95 kDa member of the Gab family of proteins. It is largely expressed in T and B cells, and serves as an adaptor molecule for the transmission of signals from cytokine and growth factor receptors. Bcr-Abl signals through Gab2, and Gab2 activates ERK. Human Gab2 is 676 amino acids (aa) in length, and contains one pleckstrin homology domain (aa 6‑117) and one methyl binding domain sequence (aa 454‑525). Multiple phosphorylation sites exist, with S159 being an important regulatory site. One alternate start site is reported at M39. Over aa 82‑292, human Gab2 is 93% and 98% aa identical to mouse and canine Gab2, respectively.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Gab2
Gln82-Phe292
Accession # Q9UQC2
Gln82-Phe292
Accession # Q9UQC2
Specificity
Detects endogenous human Gab2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Gab2 Antibody
Detection of Human Gab2 by Western Blot.
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human Gab2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4877) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gab2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human Gab2 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line
Sample: MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GAB2
Long Name
GRB2-associated Binding Protein 2
Alternate Names
p100
Gene Symbol
GAB2
UniProt
Additional GAB2 Products
Product Documents for Human Gab2 Antibody
Product Specific Notices for Human Gab2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
