Human GAB3 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB7127S Datasheet / COA / SDS

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FAB7127S-100UG

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Human GAB3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAB-3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 720306

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human GAB3
Leu121-Asn263
Accession # Q8WWW8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GAB3

GAB3 (GRB2-associated-binding protein 3) is a ~75-80 kDa member of the Gab family of scaffolding proteins which show overlapping expression in hematopoietic cells. All contain an N-terminal pleckstrin homology domain and multiple tyrosine phosphorylation and SH2 and SH3 domain binding sites. M-CSF stimulation of myeloid precursors through M-CSF R co-induces GAB3 and the associated adaptor protein GRAP2, and causes macrophage differentiation associated with tyrosine phosphorylation of GAB3. Over amino acids (aa) 121-263, human GAB3 shares 73% aa identity with mouse GAB3. A potential isoform of 548 aa lacks aa 509-548, while another of 488 aa lacks aa 173-356 and diverges at aa 475. A third potential isoform of 206 aa has an alternate start site at aa 380.

Long Name

GRB2-associated Binding Protein 3

Entrez Gene IDs

139716 (Human); 210710 (Mouse)

Alternate Names

DOS/Gab family member 3; Gab3 scaffolding protein; GAB3; GRB2-associated binder 3; GRB2-associated binding protein 3; GRB2-associated-binding protein 3; Growth factor receptor bound protein 2-associated protein 3