Human GADD45 alpha Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5510R Datasheet / COA / SDS

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AF5510R-100UG

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All GADD45 alpha Antibodies

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Human GADD45 alpha Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GADD45 alpha in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GADD45Î±
Met1-Arg165
Accession # P24522

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GADD45 alpha

GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.

Long Name

Growth Arrest and DNA Damage Gene alpha

Entrez Gene IDs

1647 (Human); 13197 (Mouse); 25112 (Rat)

Alternate Names

DDIT-1; DDIT1growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; GADD45 alpha; GADD45a; GADD45DNA-damage-inducible transcript 1; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible, alpha