Human Girdin Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5345N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5345N-100UG

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Human Girdin Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Girdin in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Girdin
Asn1510-Leu1824
Accession # Q3V6T2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Girdin

Girdin (GIRDers of actIN filaments; also APE and GIV) is a member of the CCDC88 family of molecules. It is a cytosolic protein, ubiquitously expressed, and plays a key role in the motility of breast cancer cells. Human Girdin is 1871 amino acids (aa) in length. It contains two coiled-coil regions that mediate homodimerization (aa 196-425 and 458-1358), and one phosphoinositide-binding domain (aa 1390-1408). Normally, Girdin is membrane-associated via an interaction with phosphoinositides. Upon phosphorylation at Ser1417 by Akt, it relocates to lamellipodia where it crosslinks actin filaments. Multiple isoforms of Girdin have been detected.

Long Name

Girders of Actin Filament

Entrez Gene IDs

55704 (Human); 108686 (Mouse); 305605 (Rat)

Alternate Names

Akt phosphorylation enhancer; APE; APEGIRDIN; CCDC88A; coiled-coil domain containing 88A; Coiled-coil domain-containing protein 88A; G alpha-interacting vesicle-associated protein; G{alpha}-interacting vesicle-associated protein; Girders of actin filament; Girdin; GIV; GIVFLJ10392; GRDN; GRDNAKT-phosphorylation enhancer; HkRP1; HkRP1Galpha-interacting vesicle-associated protein; Hook-related protein 1; KIAA1212DKFZp686D0630