Human GKAP/DLGAP1 Antibody

DLGAP1 (Disks Large-associated protein 1; also SAPAP1, GKAP and SAP90 binding protein) is an intracellular, 117-130 kDa member of the SAPAP family of proteins. It is widely expressed (astrocytes, neurons, fibroblasts and epithelium), and serves as a platform for dynein-DGL1 interaction. This is important during cell migration where the centrosome must be positioned between the nucleus and direction of migration in response to microtubule formation under the protruding edge of the cell membrane. DLGAP1 first binds DLG-1 and then recruits dynein, with a resultant interaction that allows for microtubule anchoring and centrosome positioning. Human DLGAP1 is 977 amino acids (aa) in length. It contains multiple 14 aa acidic repeats, three Pro-rich segments, and at least 14 potential phosphorylation sites. Multiple potential splice variants are reported. In various combinations, there may be a 17, 27 and 31 aa substitution for aa 1-319, a deletion of either aa 531-540 or 531-558, a 24 aa substitution for aa 1-318, and a 13 aa substitution for aa 531-977. Most appear to generate 70-80 kDa isoforms in SDS-PAGE. Over aa 368-472, human and mouse DLGAP1 are identical in aa sequence.