Human GPR111 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF6494R Datasheet / COA / SDS

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AF6494R-100UG

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Human GPR111 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR111 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) GPR30, rhGPR56, rhGPR114, rhGPR115, rhGPR124, and rhGPR125 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GPR111
Cys19-Ser375
Accession # NP_722581

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GPR111

GPR111 (G-protein coupled receptor 111; also PRG20) is a 71 kDa (predicted), seven transmembrane (TM) member of the GPR-2 family, LN-7TM subfamily of molecules. It is reportedly expressed in lung, mammary gland and diencephalon. Human GPR111 is 642 amino acids (aa) in length. It contains an extended N-terminal extracellular region with a mucin like stalk (aa 1-383), followed by a series of seven TM domains and a short C-terminal cytoplasmic tail. The N-terminus possesses a GPS (GPCR proteolytic site) (aa 324-368) that likely generates a soluble cleavage product. GPR111 is considered an adhesion-type GPCR, and as such, is expected to form dimers, if not oligomers. There is one potential splice variant for GPR111. It shows a 92 aa substitution for aa 1-24 coupled to a 19 aa substitution for aa 622-642. Over aa 19-375, human GPR111 shares 68% aa identity with mouse GPR111.

Long Name

G-Protein-Coupled Receptor 111

Entrez Gene IDs

222611 (Human); 435529 (Mouse); 301269 (Rat)

Alternate Names

G protein-coupled receptor 111; GPR111; PGR20