Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all GRK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRK3
Pro469-Leu688
Accession # P35626

Specificity

Detects human GRK3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human GRK3 Antibody

Detection of Human GRK3 antibody by Western Blot.

Detection of Human GRK3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GRK3 antibody by Simple WesternTM.

Detection of Human GRK3 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK3 at approximately 80-81 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human GRK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4785) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human GRK3 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRK3

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK3 (also known as beta -adrenergic receptor kinase 2, or beta ARK2) and GRK2 ( beta ARK1) are members of the beta -adrenergic receptor kinase subfamily. GRK3 may phosphorylate alpha -synuclein at S129, suggesting a potential involvement for this kinase in Parkinson’s disease.

Long Name

G protein-coupled Receptor Kinase 3

Alternate Names

ADRBK2, BARK2, Beta-ARK-2

Entrez Gene IDs

157 (Human); 320129 (Mouse); 25372 (Rat)

Gene Symbol

GRK3

UniProt

P35626

Additional GRK3 Products

Product Documents for Human GRK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GRK3 Antibody

For research use only

Related Research Areas

Intracellular Kinases

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