Human HIPK1 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5617V Datasheet / COA / SDS

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AF5617V-100UG

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Human HIPK1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human HIPK1 in Western blots. Approximately 15% cross reactivity to HIPK2 is observed in Western blot.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HIPK1
Gln701-Ser900
Accession # Q86Z02

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HIPK1

HIPK1 (Homeodomain-interacting protein kinase 1) is a 120‑130 kDa member of the HIPK subfamily, CMGC Ser/Thr family of protein kinases. It is ubiquitously expressed, and plays a role in transcriptional regulation and signal transduction. Human HIPK1 is 1210 amino acids (aa) in length. It contains a protein kinase domain (aa 190‑518) that phosphorylates itself as well as Daxx and p53, an interaction region between aa 581‑847, a PEST sequence (aa 889‑973) and a YH domain (aa 1066‑1209) that recruits CtBP. Four different splice variants have been identified. Over aa 701‑900, human and mouse HIPK1 share 97% aa identity.

Long Name

Homeodomain Interacting Protein Kinase 1

Entrez Gene IDs

204851 (Human); 15257 (Mouse); 365895 (Rat)

Alternate Names

EC 2.7.11.1; HIPK1; homeodomain interacting protein kinase 1; homeodomain interacting protein kinase 1-like protein; homeodomain-interacting protein kinase 1; KIAA0630MGC26642; MGC33446; MGC33548; Myak; Nbak2; nuclear body associated kinase 2b