Human HPRG Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB1869V Datasheet / COA / SDS

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FAB1869V-100UG

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Human HPRG Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HPRG in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Cystatin B, C, D, E/M, S, A, SA, SN, rhFetuin A, B, rmHPRG, rhKininogen, or rhKininostatin.

Source

Monoclonal Mouse IgG2b Clone # 227901

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HPRG
Val19-Lys525
Accession # P04196

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HPRG

Human histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67 - 75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525) (3, 4). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn⁺⁺-dependent manner, it is converted to plasmin by tPA (7 - 9). HPRG also shows antiangiogenic activity on endothelial cells (10).

Long Name

Histidine-rich Glycoprotein Precusor

Entrez Gene IDs

3273 (Human)

Alternate Names

DKFZp779H1622; Histidine-proline-rich glycoprotein; histidine-rich glycoprotein; HPRG; HPRGHRGP; HRG