Human HSP90 alpha Affinity Purified Polyclonal Ab

Name: Human HSP90 alpha Affinity Purified Polyclonal Ab
Brand: R&D Systems
SKU: A-440
Price: 202 USD

Catalog #: A-440 Datasheet / COA / SDS

Product is in the process of being discontinued

Discontinued Product

A-440 has been discontinued.

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Detection of Human HSP90 alpha by Western Blot.

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All HSP90 alpha Antibodies

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Human HSP90 alpha Affinity Purified Polyclonal Ab Summary

Species Reactivity

Human

Source

Polyclonal Rabbit IgG

Immunogen

Purified, recombinant human HSP90 alpha protein
Accession # P07900

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Applications

Recommended Concentration

Sample

Western Blot

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HSP90 alpha by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP90a Antigen Affinity-purified Polyclonal Antibody (Catalog # A-440) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP90a at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HSP90 alpha

The heat shock protein-90 kDa (HSP90) is a composite name for a large group of genes whose molecular weights average 90 kDa. HSP90 functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. In addition to its role as a molecular chaperone and stress response protein, HSP90 is a central component in a number of cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved, and accounts for 1-2% of the total cellular protein. Eukaryotic cells have two principal forms of HSP90 including the inducible HSP90 alpha (also known as HSP90AA1, HSP90A, HSPC1, HSPCA and HSP86) and the constitutively expressed HSP90 beta (also known as HSP90AB1, HSP90B, HSPCB, HSPC2, and HSP89-beta. While HSP90 alpha and HSP90 beta share 90% identity, this affinity-purified polyclonal antibody is specific for HSP90 alpha.

Long Name

Heat Shock Protein 90 alpha

Entrez Gene IDs

3320 (Human); 15519 (Mouse); 299331 (Rat)

Alternate Names

FLJ31884; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein HSP 90-alpha; HSP86; Hsp89; HSP89A; HSP90 alpha; Hsp90; HSP90A; HSP90AA1; HSP90AHSP86; HSP90N; HSPC1; HSPC1HSP 86; HSPCA; HSPCAHSP89A; HSPCAL1; HSPCAL4; HSPN; LAP2; Renal carcinoma antigen NY-REN-38