Human IGFBP-4 N-terminal Antibody

Name: Human IGFBP-4 N-terminal Antibody
Brand: R&D Systems
SKU: MAB7110

Catalog #: MAB7110 Datasheet / COA / SDS

Discontinued Product

MAB7110 has been discontinued.

View all IGFBP-4 products.

Detection of Human IGFBP‑4 N-terminal Fragment by Western Blot.

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Human IGFBP-4 N-terminal Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGFBP4 N-Terminal in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 721108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

synthetic peptide from human IGFBP-4
Asp148-Met156
Accession # P22692

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IGFBP‑4 N-terminal Fragment by Western Blot. Western blot shows recombinant human IGFBP-4 N-terminal fragment (10 ng/lane) and recombinant human IGFBP-4 C-terminal fragment (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGFBP-4 N-terminal Monoclonal Antibody (Catalog # MAB7110) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGFBP-4 N-terminal fragment at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-4

Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) precursor protein with a predicted 21 aa signal peptide that is processed to generate the 237 aa mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% aa sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, -4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino‑ and carboxy-terminal third of the molecule. IGFBPs have been shown to either inhibit or enhance the biological activities of IGF, or act in an IGF-independent manner. Post-translational modification of IGFBPs, including phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP-4 can be cleaved at Met156-Lys157.

References

Jones, J. I. and D.R. Clemons (1995) Endocrine Rev. 16:3.
Kelly, K.M. et al. (1996) Intl. J. Biochem. Cell Biol. 28:619.
Kim, H-S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12981.

Long Name

Insulin-like Growth Factor Binding Protein 4

Entrez Gene IDs

3487 (Human); 16010 (Mouse)

Alternate Names

BP-4; HT29-IGFBP; IBP4insulin-like growth factor-binding protein 4; IGF-binding protein 4; IGFBP4; IGFBP-4; IGFBP-4IBP-4; insulin-like growth factor binding protein 4