Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ig Lambda C Domain Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IGLC2
Gln2-Ser106
Accession # P0CG05

Specificity

Detects recombinant human IGLC2 in direct ELISAs and Western blots. Detects endogenous Immunoglobulin lambda constant region in natural samples by Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Ig lambda C Domain Antibody

Detection of Human Immunoglobulin Lambda Constant Region antibody by Western Blot.

Detection of Human Immunoglobulin Lambda Constant Region by Western Blot.

Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Ig lambda C Domain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ig Lambda C Domain

Immunoglobulin lambda constant region is a Ke-Oz- component of immunoglobulin (Ig) lambda light chains and is found C-terminal to the joining region of the light chain. IGLC2 is 105 amino acids (aa) in length. It contains one Ig-like domain (aa 7-100) and generates a disulfide bond with an IgH chain via Cys104. One potential variant shows a three aa substitution for aa 7-9. The closest mouse  lambda ortholog shares 61% aa identity with full-length human IGLC2. IGLC2 shares 93 - 98 % aa identity with IGLC1, IGLC3 and IGLC7.

Long Name

Immunoglobulin lambda C Domain

Alternate Names

IGLC, IGLC2

Entrez Gene IDs

3538 (Human)

Gene Symbol

IGLC2

UniProt

P0CG05 (Human)

Additional Ig Lambda C Domain Products

Product Documents for Human Ig lambda C Domain Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ig lambda C Domain Antibody

For research use only

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