Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 578410
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # Q6ZMJ4

Specificity

Detects human IL-34 in sandwich immunoassays.  In this format, no cross-reactivity with recombinant mouse IL-34 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human IL‑34 Antibody

Human IL-34 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑34 Biotinylated Antibody (Catalog # BAM52652)
  • Standard: Recombinant Human IL-34 Protein (Catalog # 5265-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 2 using MAB52651 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-34

Interleukin 34 (IL-34) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. The mature chain contains one potential site for N-linked glycosylation. IL-34 is widely expressed, but it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF‑1R). Functionally, IL-34 stimulates monocyte viability.

Long Name

Interleukin 34

Alternate Names

C16orf77, IL34

Entrez Gene IDs

146433 (Human); 76527 (Mouse)

Gene Symbol

IL34

UniProt

Additional IL-34 Products

Product Documents for Human IL‑34 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL‑34 Antibody

For research use only

Related Research Areas

Customer Reviews for Human IL‑34 Antibody (1)

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  • Human IL-34 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human IL‑34 Antibody MAB52651

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