Human Integrin alpha 4/CD49d Antibody

Name: Human Integrin alpha 4/CD49d Antibody
Brand: R&D Systems
SKU: BBA37
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # BBA37

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Citations (20)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Adhesion Blockade

Cited:

Neutralization, Flow Cytometry, Immunocytochemistry, Blocking

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 2B4

View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

U937 human histiocytic lymphoma cell line

Specificity

Detects human Integrin alpha 4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication 2:87).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Integrin alpha 4/CD49d Antibody

Application

Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 10⁴ cells/well) to immobilized recombinant human VCAM-1 (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 5 μg/mL of the antibody.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d, is a transmembrane protein that forms heterodimers with beta 1 or beta 7 integrins. These complexes contribute to the trafficking of many hematopoietic cell types. Integrins alpha 4 beta 1 (also known as VLA-4) and alpha 4 beta 7 (LPAM-1) are receptors for fibronectin, VCAM and MAdCAM-1.