Human IRAK2 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB6690G Datasheet / COA / SDS

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FAB6690G-100UG

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Human IRAK2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRAK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IRAK1, 3, or 4 is observed. In Western blots, less than 25% cross-reactivity with rhIRAK1, 3, and 4 is observed.

Source

Monoclonal Mouse IgG3 Clone # 600848

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human IRAK2
Ala2-Asn590
Accession # O43187

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRAK2

IRAK2 is an approximately 75 kDa, 625 amino acid (aa) member of the IL-1 receptor-associated kinase (IRAK) family. The four IRAKs are ser/thr kinases that regulate signaling from Toll-like and IL-1 receptors. IRAKs contain N-terminal death domains and central kinase domains, but activity of the IRAK2 kinase domain is unknown. IRAK1 provides initial, and IRAK2 sustained, NFkB activation and proinflammatory cytokine production in macrophages, acting downstream of IRAK4. In the region used as an immunogen, human IRAK2 shares 69% and 70% amino acid identity with mouse and rat IRAK2, respectively.

Long Name

Interleukin-1 Receptor-Associated Kinase 2

Entrez Gene IDs

3656 (Human); 108960 (Mouse); 362418 (Rat)

Alternate Names

interleukin-1 receptor associated kinase-2; interleukin-1 receptor-associated kinase 2; interleukin-1 receptor-associated kinase-like 2; IRAK2; IRAK-2; MGC150550