Interferon Regulatory Factor 2 (IRF2) belong to the interferon regulatory transcription factor family. IRF2 functions as a regulator of type I interferons influencing cellular proliferation and immune response through transcriptional regulation. IRF2 competitively inhibits IRF1 gene activation, as well as, stimulating transcription on its own. Over-expression of IRF2 in NIH3T3 cells leads to transformation, whereas, loss of IRF2 expression in knock out mice leads to a variety of abnormalities including loss of NK cells due to increased apoptosis.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human IRF2
Val151-Cys349
Accession # P14316
Val151-Cys349
Accession # P14316
Specificity
Detects human IRF2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IRF2 Antibody
Detection of Human IRF2 by Western Blot.
Western blot shows nuclear extracts of LNCaP human prostate cancer cell line, U937 human histiocytic lymphoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IRF2 by Simple WesternTM.
Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IRF2 at approximately 61 kDa (as indicated) using 5 µg/mL of Goat Anti-Human IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4049) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Applications for Human IRF2 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Western Blot
0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line chronic myelogenous leukemia, HT‑29 human colon adenocarcinoma cell line, and MCF‑7 human breast cancer cell line
Sample: K562 human chronic myelogenous leukemia cell line chronic myelogenous leukemia, HT‑29 human colon adenocarcinoma cell line, and MCF‑7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IRF2
Long Name
Interferon Regulatory Factor 2
Alternate Names
DKFZp686F0244, interferon regulatory factor 2, IRF-2
Gene Symbol
IRF2
UniProt
Additional IRF2 Products
Product Documents for Human IRF2 Antibody
Product Specific Notices for Human IRF2 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
