Human ITM2A Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4876R Datasheet / COA / SDS

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AF4876R-100UG

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Human ITM2A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITM2A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ITM2B is observed, and less than 1% cross-reactivity with rhITM2C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ITM2A
Pro80-Glu263
Accession # O43736

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ITM2A

ITM2A (Integral membrane protein 2A; also protein E25) is a 43-45 kDa member of the ITM2 family of proteins. It is expressed by chondrocytes involved in endochondrial ossification, skeletal muscle, adipose tissue-derived stem cells, and CD3-activated CD4⁺ and CD8⁺ T cells. ITM2A is found embedded in both plasma membrane and Golgi complex. Human ITM2A is a 263 amino acid (aa) type II transmembrane glycoprotein. The ECD (aa 75-263) contains one BRICHOS domain (aa 133‑227). Proteolytic cleavage by furin is typical for BRICHOS domain-containing proteins. ITM2A may exist as a dimer. There are two potential splice variants, one that shows a deletion of aa 55-81, and a second that shows a deletion of aa 38-81. Full-length human ITM2A shares 95% aa identity with mouse ITM2A.

Long Name

Integral Membrane Protein 2A

Entrez Gene IDs

9452 (Human); 16431 (Mouse); 317218 (Rat)

Alternate Names

BRICD2A; BRICHOS domain containing 2A; E25A; E25ABRICD2A; integral membrane protein 2A; ITM2A; Protein E25