Human KLF10 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF7016S Datasheet / COA / SDS

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AF7016S-100UG

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All KLF10 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF10 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLF10 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human KLF12 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KLF10
Asn31-Ser154
Accession # Q13118

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: KLF10

KLF10 (Kruppel-like factor 10; also TIEG1 and EGR-alpha ) is a 55-65 kDa member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-beta, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-beta signaling. Human KLF10 is 480 amino acids (aa) in length and contains three C2H2-type zinc-finger motifs (aa 369-451). There is one potential alternate splice form that shows a deletion of aa 2-11. Over aa 31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.

Long Name

Kruppel-Like Factor 10

Entrez Gene IDs

7071 (Human); 21847 (Mouse); 81813 (Rat)

Alternate Names

EGRA; EGR-alpha; KLF10; Krueppel-like factor 10; Kruppel-like factor 10; TGFB inducible early growth response; TGFB-inducible early growth response protein 1; TIEG1; TIEG-1; TIEG1TIEG; Transforming growth factor-beta-inducible early growth response protein 1