Human Lactase-like Protein/LCTL Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5984N Datasheet / COA / SDS

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AF5984N-100UG

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Human Lactase-like Protein/LCTL Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Lactase-like Protein/LCTL
Arg23-Glu541
Accession # Q6UWM7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Lactase-like Protein/LCTL

LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.

Entrez Gene IDs

197021 (Human); 235435 (Mouse); 315750 (Rat)

Alternate Names

FLJ33279; KLG; klotho gamma; Klotho/lactase-phlorizin hydrolase-related protein; KLPH; KLPHKL lactase phlorizin hydrolase; Lactase like Protein; Lactase-like Protein; lactase-like; LCTL