Human Latent TGF-beta bp1 Antibody (35409)
R&D Systems | Catalog # MAB388
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Western Blot, Immunoaffinity Purification
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation, ELISA Detection
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 35409
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Product Specifications
Immunogen
Human platelet-derived large latent TGF-beta 1 complex
Specificity
Detects human LTBP-1 from the large latent TGF-beta 1 complex.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Latent TGF-beta bp1 Antibody (35409)
Detection of Latent TGF¿ beta bp1 by Western Blot
LTBP-1 accumulates in CADASIL brain vessels and co-localizes with Notch3-ECD aggregates. a Frozen brain sections from a CADASIL patient and a healthy control containing an arteriole (left panels) or a capillary (right panels) were stained for LTBP-1 and analyzed by fluorescence microscopy. b Frozen brain sections of three CADASIL patients were stained for LTBP-1 and Notch3-ECD and analyzed by confocal fluorescence microscopy. c LTBP-1 co-fractionates with Notch3-ECD. The final ( beta -ME-containing) fractions of sequentially extracted brain vessels from CADASIL patients and controls were immunoblotted for Notch3-ECD, LTBP-1 and beta -tubulin. Shown is a representative blot from two independent experiments. (CAD: CADASIL patient; Ctrl: control). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25190493), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Latent TGF-beta bp1 Antibody (35409)
Application
Recommended Usage
Immunoaffinity Purification
This antibody can be used to affinity purify the large latent TGF-beta complex (containing both the 230 and 195 kDa binding protein) and will also pull out “free” binding protein that is not associated with the small latent TGF-beta.
Western Blot
1 µg/mL
Sample: Large latent TGF-beta complex from human platelets
under non-reducing conditions only
Sample: Large latent TGF-beta complex from human platelets
under non-reducing conditions only
Reviewed Applications
Read 3 reviews rated 3.7 using MAB388 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Latent TGF-beta bp1
References
- Miyazono, K. et al. (1991) EMBO J. 10:1091.
- Moren, A. et al. (1994) J. Biol. Chem. 269:32469.
- Yin, W. et al. (1995) J. Biol. Chem. 270:10147.
- Kanzaki, T. et al. (1990) Cell 61:1051.
- Saharinen, J. et al. (1998) J. Biol. Chem. 273:18459.
Alternate Names
Latent TGFbeta bp1, LTBP1
Entrez Gene IDs
4052 (Human)
Gene Symbol
LTBP1
Additional Latent TGF-beta bp1 Products
Product Documents for Human Latent TGF-beta bp1 Antibody (35409)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Latent TGF-beta bp1 Antibody (35409)
For research use only
Citations for Human Latent TGF-beta bp1 Antibody (35409)
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Customer Reviews for Human Latent TGF-beta bp1 Antibody (35409) (3)
3.7 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 02/07/2020Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/14/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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