Human MAP3K8/Tpl2/COT Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB4586R Datasheet / COA / SDS

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FAB4586R-100UG

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Human MAP3K8/Tpl2/COT Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MAP3K8/Tpl2/COT in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 634130

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human MAP3K8/Tpl2/COT
Gln173-Gln395
Accession # P41279

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MAP3K8/Tpl2/COT

MAP3K8, also called Tpl2 (tumor progression locus 2) or COT (Cancer Osaka Thyroid oncogene) is a cytosolic Ser/Thr kinase. MAP3K8 is one of the MAP kinase kinase (MAPKK) kinases that regulates the ERK1/ERK2 pathway in response to IL-1, facilitating IL-8 and MIP-1 beta production. MAP3K8 activates I kappa B kinases and induces the nuclear production of NF-kappa B. Human MAP3K8 shares 97% and 98% amino acid (aa) identity with mouse and rat MAP3K8, respectively, within the region used as an immunogen. Alternative start sites can give a 58 kDa (467 aa) isoform with stronger activity but shorter half-life than a 52 kDa (397 aa) isoform.

Long Name

Mitogen-activated Protein Kinase Kinase Kinase 8

Entrez Gene IDs

1326 (Human); 26410 (Mouse); 116596 (Rat)

Alternate Names

Cancer Osaka thyroid oncogene; c-COT; cot (cancer Osaka thyroid) oncogene; COT; COTMEKK8; EC 2.7.11.25; EST; ESTF; ESTFLJ10486; Ewing sarcoma transformant; MAP3K8; mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL2; Tpl-2; Tumor progression locus 2; tumor progression locus-2