Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all MAP4K5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MAP4K5
Lys395-Asn484 (Asn473Lys)
Accession # Q9Y4K4

Specificity

Detects human MAP4K5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human MAP4K5 Antibody

Detection of Human MAP4K5 antibody by Western Blot.

Detection of Human MAP4K5 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human MAP4K5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MAP4K5 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human MAP4K5 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, HT1080 human fibrosarcoma cell line, and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 1 review rated 5 using AF8007 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAP4K5

MAP4K5 (MAP Kinase Kinase Kinase Kinase 5; also known as KHS, MEKKK5, and GCKR) is a 94-96 kDa member of the STE (STErile 20 protein) subfamily, STE Ser/Thr protein kinase family, protein kinase superfamily of enzymes. It is ubiquitously expressed, and serves as an "intermediary" between GPCRs and cytosolic MAP kinases. It also interacts with Crk (CT10 Regulator of Kinase)-related adaptor molecules, facilitating the formation of signaling complexes around Tyr phosphorylated molecules. And finally, it represents an upstream component of a TNFR1 signaling pathway that requires activation of stress-activated protein kinases. Human MAP4K5 is 846 amino acids 9aa) in length. It contains a 258 aa kinase domain (aa 20-277) that is coupled to a 316 aa CNH (citron homology) domain (aa 506-826) which typically binds GTPases. There are two isoform variants. One shows a deletion of both Pro481 and Glu569, while another possesses a substitution of 16 aa for aa 569-846. Over aa 395-484, human MAP4K5 shares 83% aa sequence identity with mouse MAP4K5.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

GCKR, KHS1, MAPKKKK5

Entrez Gene IDs

11183 (Human); 399510 (Mouse); 503027 (Rat)

Gene Symbol

MAP4K5

UniProt

Q9Y4K4

Additional MAP4K5 Products

Product Documents for Human MAP4K5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MAP4K5 Antibody

For research use only

Customer Reviews for Human MAP4K5 Antibody (1)

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  • Human MAP4K5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 04/26/2017
    Human MAP4K5 Antibody AF8007

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