Human MBD3 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3839G Datasheet / COA / SDS

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AF3839G-100UG

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Human MBD3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MBD3 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MBD3 isoform 2
Met1-Val259
Accession # AAH43619

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MBD3

MBD3 (methyl-CpG-binding domain protein # 3) is a 38‑40 kDa member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD2. Human MBD3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa’s 1‑72) and a C-terminal coiled-coil region (aa’s 216‑245) that may be involved in protein-protein interaction. Human MBD3 shares 95% aa identity with mouse, rat, and canine MBD3, with differences essentially limited to the C‑terminal 25 amino acids.

Long Name

Methyl-CpG Binding Domain Protein 3

Entrez Gene IDs

53615 (Human); 17192 (Mouse); 314628 (Rat)

Alternate Names

MBD3; methyl-CpG binding domain protein 3; methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3