Human MFNG Alexa Fluor® 532-conjugated Antibody

Catalog #: AF6355X Datasheet / COA / SDS

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AF6355X-100UG

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Human MFNG Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MFNG in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MFNG
Gly37-Arg321
Accession # O00587

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MFNG

MFNG (Manic fringe N-acetylglucosaminyltransferase; also O-fucosylpeptide 3-beta -N-acetylglucosaminyltransferase) is a 52 - 55 kDa member of the glucosyltransferase 31 family. It is a Golgi membrane protein that transfers N-acetylglucosamine to an O-linked fucose residue on Notch. Activity on Notch increases Delta-1 induced signaling while suppressing Jagged-1 signaling. MFNG is found in fetal pancreatic endocrine progenitor cells and immature ventricular zone neurons. Human MFNG is a 321 amino acid (aa) type II transmembrane protein. It contains a short 7 aa cytoplasmic region, plus a 294 aa luminal domain (aa 28-321). There are two potential splice variants, one that shows a 15 aa substitution for aa 104-321, and another that contains a three aa substitution for aa 86-102. Over aa 37-321, human MFNG shares 85% aa identity with mouse MFNG.

Long Name

Manic Fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Entrez Gene IDs

4242 (Human)

Alternate Names

beta-1,3-N-acetylglucosaminyltransferase manic fringe; EC 2.4.1.222; manic fringe (Drosophila) homolog; manic fringe homolog (Drosophila); MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; MFNG; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase