Human Mind Bomb 2/MIB2 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB72891U Datasheet / COA / SDS

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FAB72891U-100UG

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Human Mind Bomb 2/MIB2 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 739505

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Mind Bomb 2/MIB2
Ala418-Lys520
Accession # Q96AX9

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mind Bomb 2/MIB2

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Long Name

Mindbomb Homolog 2

Entrez Gene IDs

142678 (Human); 76580 (Mouse)

Alternate Names

E3 ubiquitin-protein ligase MIB2; EC 6.3.2; EC 6.3.2.-; EC 6.3.2.13; FLJ20648; FLJ41406; MIB2; Mind Bomb 2; Mind bomb homolog 2; Mind Bomb-2; mindbomb homolog 2 (Drosophila); Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; SKD; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; ZZ type with ankyrin repeat domain 1; ZZANK1; ZZZ5