Human/Mouse Adiponutrin/PNPLA3 Antibody

Name: Human/Mouse Adiponutrin/PNPLA3 Antibody
Brand: R&D Systems
SKU: AF5179

Catalog #: AF5179 Datasheet / COA / SDS

Discontinued Product

AF5179 has been discontinued.

View all Adiponutrin/PNPLA3 products.

Detection of Human and Mouse Adiponutrin/PNPLA3 by Western Blot.

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All Adiponutrin/PNPLA3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Adiponutrin/PNPLA3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Adiponutrin/PNPLA3 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Adiponutrin/PNPLA3
Arg160-Arg327
Accession # Q91WW7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Adiponutrin/PNPLA3 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells, mouse liver tissue, human brain tissue, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Adiponutrin/PNPLA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/PNPLA3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponutrin/PNPLA3

Mouse adiponutrin (also patatin-like phospholipase domain-containing protein 3) is a 45-48 kDa member of the adiponutrin family of phospholipase A2 enzymes. It is expressed in white and brown adipocytes, hepatocytes and brain. Adiponutrin is reduced in amount during fasting, and appears to act as an acute sensor of the body energy stores. Based on other family members, mouse adiponutrin is a type II transmembrane protein that is 413 amino acids (aa) in length. It contains one patatin domain (aa 10-179) that is associated with enzyme activity. There appear to be three splice variants. One shows a 69 aa substitution for aa 384-413, a second shows a deletion of aa 385-413, while a third shows a three aa substitution for aa 384-413. Over aa 160-327, mouse adiponutrin shares 84% and 62% aa identity with rat and human adiponutrin, respectively; and over aa 161-229, mouse adiponutrin shares 90% aa identity with human adiponutrin.

Long Name

Patanin-like Phospholipase Domain-containing Protein 3

Entrez Gene IDs

80339 (Human); 362972 (Rat)

Alternate Names

Acylglycerol O-acyltransferase; Adiponutrin; ADPN; ADPNdJ796I17.1; C22orf20; Calcium-independent phospholipase A2-epsilon; chromosome 22 open reading frame 20; EC 2.3.1.-; EC 2.7.7.56; EC 3.1.1.3; EC 4.2.3.4; FLJ22012; iPLA(2)epsilon; iPLA2-epsilon; patatin-like phospholipase domain containing 3; patatin-like phospholipase domain-containing protein 3; PNPLA3