Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Glu522-Thr696
Accession # Q8CB25
Specificity
Clonality
Host
Isotype
Scientific Data Images for Bcl-6 Antibody
Detection of Mouse and Human Bcl‑6 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Bcl‑6 by Simple WesternTM.
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑6 at approximately 111 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Bcl‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Bcl-6 Antibody
Simple Western
Sample: Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line
Western Blot
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Bcl-6
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Bcl-6 Products
Product Documents for Bcl-6 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Bcl-6 Antibody
For research use only
Related Research Areas
Citations for Bcl-6 Antibody
Powered by Bioz
Customer Reviews for Bcl-6 Antibody
There are currently no reviews for this product. Be the first to review Bcl-6 Antibody and earn rewards!
Have you used Bcl-6 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 for a review with an image
$10/€7/£6/$10CAN/¥1110 for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
