Human/Mouse Bcl-w Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB8241U Datasheet / COA / SDS

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FAB8241U-100UG

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All Bcl-w Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bcl-w Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bcl‑w. Neutralizes recombinant human and mouse Bcl-w, but not recombinant human Bcl‑2 or Bcl‑x.

Source

Monoclonal Mouse IgG1 Clone # 90336

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Bcl-w
Met1-Thr172
Accession # Q92843

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Bcl-w

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

Long Name

B Cell Lymphoma/Leukemia w

Entrez Gene IDs

599 (Human); 12050 (Mouse)

Alternate Names

Apoptosis regulator Bcl-W; BCL2L2; Bcl2-L-2; BCL2-like 2; Bclw; Bcl-w; BCLWbcl-2-like protein 2; KIAA0271BCL2-L-2