beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

R&D Systems | Catalog # AF6664

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all beta-1,4-Glucuronyltransferase 1/B4GAT1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human beta -1,4-Glucuronyltransferase 1/B4GAT1
Asp43-Cys415
Accession # O43505

Specificity

Detects human and mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 in Western blot. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) B4GalT1 and rhB3GNT 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Detection of Human and Mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 antibody by Western Blot.

Detection of Human and Mouse beta -1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot.

Western blot shows lysates of human muscle tissue and mouse muscle tissue. PVDF Membrane was probed with 1 µg/mL of Human beta -1,4-Glucuronyltransferase 1/B4GAT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for beta -1,4-Glucuronyltransferase 1/B4GAT1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human muscle tissue and mouse muscle tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B3GNT1 ( beta -1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the beta 3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Long Name

beta-1,4-Glucuronyltransferase 1

Alternate Names

B3GNT1, iGAT, iGNT, MDDGA13

Entrez Gene IDs

11041 (Human); 108902 (Mouse); 293667 (Rat)

Gene Symbol

B4GAT1

UniProt

O43505 (Human)

Additional beta-1,4-Glucuronyltransferase 1/B4GAT1 Products

Product Documents for beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

Customer Reviews for beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

There are currently no reviews for this product. Be the first to review beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody and earn rewards!

Have you used beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies