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Human/Mouse BLIMP1/PRDM1 Antibody

Catalog #: AF3608
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3608
AF3608-SP

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Detection of Human BLIMP1/PRDM1 by Western Blot.
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Citations (2)
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Human/Mouse BLIMP1/PRDM1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BLIMP1/PRDM1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BLIMP1/PRDM1
Leu687-Pro789
Accession # AAO45623
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BLIMP1/PRDM1 antibody by Western Blot. View Larger

Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BLIMP1/PRDM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3608) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BLIMP1/PRDM1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLIMP1/PRDM1

BLIMP-1 is a 97 kDa multidomain transcription repressor that has a SET domain in its N-terminal region and four C2H2-type zinc fingers in its C-terminal regions. It promotes terminal differentiation of B cells into plasma cells and also has an important role in primordial germ cell specification. An alternative splice variant that lacks 101 N-terminal amino acid (aa) residues of full-length BLIMP1 has been reported. Over the region used as immunogen, human and mouse BLIMP1 share 89% aa sequence identity.

Long Name
B Lymphocyte Induced Maturation Protein
Entrez Gene IDs
639 (Human); 12142 (Mouse); 309871 (Rat)
Alternate Names
Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1

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Citations for Human/Mouse BLIMP1/PRDM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A role for Lin28 in primordial germ-cell development and germ-cell malignancy.
    Authors: West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ
    Nature, 2009-07-05;460(7257):909-13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
    Authors: J Taranda, G Mathew, K Watrud, N El-Amine, MF Lee, C Elowsky, A Bludova, S Escobar Av, DG Nowak, TL Wee, JE Wilkinson, LC Trotman, P Osten
    Cell Reports, 2021-11-16;37(7):110027.

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