Carbonic Anhydrase VIII/CA8 Antibody (308320)

R&D Systems | Catalog # MAB2187

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 308320
View all Carbonic Anhydrase VIII/CA8 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219

Specificity

Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Carbonic Anhydrase VIII/CA8 Antibody (308320)

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 antibody by Western Blot.

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody (Catalog # MAB2187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Carbonic Anhydrase VIII/CA8 Antibody (308320)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human brain (cerebellum) tissue and mouse brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Carbonic Anhydrase VIII/CA8

Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
  3. Hirota, J. et al. (2003) Biochem. J. 372: 435.
  4. Miyaji, E. et al. (2003) J. Pathol. 201: 37.
  5. Lu, S.H. et al. (2004) Lung Cancer 44: 273.

Alternate Names

CA8, CALS, CARP

Entrez Gene IDs

767 (Human)

Gene Symbol

CA8

UniProt

P35219

Additional Carbonic Anhydrase VIII/CA8 Products

Product Documents for Carbonic Anhydrase VIII/CA8 Antibody (308320)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Carbonic Anhydrase VIII/CA8 Antibody (308320)

For research use only

Customer Reviews for Carbonic Anhydrase VIII/CA8 Antibody (308320)

There are currently no reviews for this product. Be the first to review Carbonic Anhydrase VIII/CA8 Antibody (308320) and earn rewards!

Have you used Carbonic Anhydrase VIII/CA8 Antibody (308320)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies