EED Antibody

Detection of Human EED by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HT-29 human colon adenocarcinoma cell line, human colon cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EED Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5827) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EED at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of EED by Western Blot

PROTAC-6272 selectively degrades PRC2 via VHL-mediated ubiquitination.A Scatter plot of protein expression changes based on global quantitative proteomics. C4-2B cells and 22Rv1 cells were treated with 0.1 µM of PROTAC-6272 for 6 h. Protein lysates were collected and subjected to global quantitative proteomics analysis as described in the experimental section. Data are presented as fold-change normalized to DMSO-treated cells. B LNCaP, 22Rv1, and VCaP cells were treated with DMSO, the negative control PROTAC-6286 (0.5 µM), PROTAC-6272 (0.5 µM), and EPZ-6438 (0.5 µM) for 48 h and then subjected to immunoblotting analysis. C C4-2B cells were treated with 20 μM proteasome inhibitor (MG-132) for 6 h, then treated with DMSO, negative control PROTAC-6286 (0.1 µM), and PROTAC-6272 (0.1 µM) for 24 h before immunoblotting. D C4-2B cells were treated with control (siCtrl) or siVHL for 72 h, followed by treatment with DMSO, negative control PROTAC-6286 (0.1 µM), and PROTAC-6272 (0.1 µM) for 24 h, and then subjected to immunoblotting. E C4-2B cells were transfected with HA-Ubiquitin (Ub) for 24 h, then treated with negative control PROTAC-6286 (0.1 µM) and PROTAC-6272 (0.1 µM) for 24 h and subjected to co-IP by anti-EZH2, followed by immunoblotting. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41388-025-03662-z), licensed under a CC-BY license. Not internally tested by R&D Systems.