Human/Mouse FoxJ3 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5786U Datasheet / COA / SDS

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AF5786U-100UG

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All FoxJ3 Antibodies

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Human/Mouse FoxJ3 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse FoxJ3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxJ3
Met375-Tyr552
Accession # NP_055762

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FoxJ3

FoxJ3 (Forkhead box protein J3) is a 69 kDa (predicted) nuclear protein that is a Class 1 member of the Fox family of transcription factors. It is expressed in embryonic neuroectoderm, neural crest and somitic condensations, and is suggested to participate in terminal tissue differentiation. Human FoxJ3 is 622 amino acids (aa) in length. It contains one forkhead DNA binding region (aa 77‑168) plus a phosphorylation site at Ser223. There are two potential splice variants. One shows a deletion of aa 177‑210, while a second shows a 12 aa substitution for aa 177‑622. Over aa 375‑552, human FoxJ3 shares 93% aa identity with mouse FoxJ3.

Long Name

Forkhead Box J3

Entrez Gene IDs

22887 (Human); 230700 (Mouse); 313554 (Rat)

Alternate Names

forkhead box J3; forkhead box protein J3; FoxJ3; KIAA1041; MGC165036; MGC176686